We will have limited support available from November 27th and November 28th, 2025. All inquiries received during this time will be processed as quickly as staff availability allows, with normal availability beginning December 1st, 2025.  

We appreciate your understanding and patience. Happy Holidays! 

FlowJo University

Getting Started in FlowJo™ Software

New to FlowJo? No problem! We will walk you through getting your analysis set up in FlowJo and then analyzing your .fcs data.

How to Download and Sign into FlowJo™ Software

How to Install FlowJo on Mac

Workspace

Managing FlowJo Preferences

Traditional Compensation

Applying Compensation Matrix

Samples Panel

Components of the Graph Window

Gating Hierarchy

Adding Statistics

Using the T-Button in FlowJo

Layout Editor

Table Editor

Boolean Gates in FlowJo

File Formats

Using Templates

Data Scaling