Download FlowJo v10.10

Get more from your high-parameter data sets now with FlowJo™ Software v10.10 now!

For customers in China, please visit https://flowjo.bectondickinson.cn for the latest installers

Download FlowJo v10.10
MacOS Requirements
  • OS X 10.9 or later
  • 4GB of memory
  • 35.5GB available storage on macOS Sierra or later*
  • Some features require an Apple ID; terms apply.
  • Some features require a compatible internet service provider; fees may apply.
Windows Requirements
  • 1 gigahertz (GHz) or faster with 2 or more cores on a compatible 64-bit processor or System on a Chip (SoC).
  • 4 gigabyte (GB).Storage64 GB or larger storage device Note: See below under “More information on storage space to keep Windows 11 up-to-date” for more details.
  • UEFI, Secure Boot capable.

In FlowJo v10, you can benefit from:


  • Spectral Plots: Create spectral plots for selected detectors and populations
  • Spectral Population Viewer: Compare populations using spectral plots
  • BD SpectralFX™ Technology Unmixing: Perform system-aware spectral unmixing on spectral data from BD FACSDiscover™ S8 Cell Sorters
  • Advanced Scaling Preferences: Define data-driven, per cytometer scaling to more easily set transform properties
  • Cluster Explorer: Explore added functionality
    • Non-cluster populations can now be visualized in the cluster explorer
    • Clusters can be re-ordered within the heat map view
    • Heatmap columns can be re-ordered or hidden
    • Expanded heat map window
  • Table creation for MEM scores: Export MEM population names in a tabular format
  • Copy Transforms: In the compensation matrix editor users can now copy the transform from one compensation matrix to another, allowing per parameter transforms to be easily applied to another data set
  • Hyperfinder: Define populations identified by a computational approach with standard gates so that they might be sorted
  • NxN plot parameter mouseover: Display parameter combinations on NxN plots for easier viewing

For more about features and a list of known issues, see the exhaustive release notes.


  • Cluster Explorer: Explore added functionality
    • Non-cluster populations can now be visualized in the cluster explorer
    • Clusters can be re-ordered within the heat map view
    • Heatmap columns can be re-ordered or hidden
    • Expanded heat map window
  • Hyperfinder: Define populations identified by a computational approach with standard gates so that they might be sorted


  • Table creation for MEM scores: Export MEM population names in a tabular format
  • Copy Transforms: In the compensation matrix editor users can now copy the transform from one compensation matrix to another, allowing per parameter transforms to be easily applied to another data set.
  • Advanced Scaling Preferences: Define data-driven, per cytometer scaling to more easily set transform properties.
  • NxN plot parameter mouseover: Display parameter combinations on NxN plots for easier viewing.

In FlowJo v10, you can benefit from:

New Spectral Features

Spectral Plots: Create spectral plots for selected detectors and populations Spectral Population Viewer: Compare populations using spectral plots BD SpectralFX™ Technology Unmixing: Perform system-aware spectral unmixing on spectral data from BD FACSDiscover™ S8 Cell Sorters

Integrated Plugins

Cluster Explorer: Explore added functionality Non-cluster populations can now be visualized in the cluster explorer Clusters can be re-ordered within the heat map view Heatmap columns can be re-ordered or hidden Expanded heat map window Hyperfinder: Define populations identified by a computational approach with standard gates so that they might be sorted

Improved functionalities

Table creation for MEM scores: Export MEM population names in a tabular format Copy Transforms: In the compensation matrix editor users can now copy the transform from one compensation matrix to another, allowing per parameter transforms to be easily applied to another data set. Advanced Scaling Preferences: Define data-driven, per cytometer scaling to more easily set transform properties. NxN plot parameter mouseover: Display parameter combinations on NxN plots for easier viewing.

FlowJo Portal License

Updating to the latest version of FlowJo for FlowJo Portal license holders is easy! Simply download the latest version of FlowJo v10 above and sign in with your FlowJo Portal ID.

Dongle

Download and install the latest version of FlowJo v10. Your dongle will automatically authenticate all versions of v10. Users with Mac OS Ventura must use v10.8.2.

Serial Number

Download and install the latest version of FlowJo v10. Your serial number will transfer and automatically authenticate all versions of v10.

 Updating to the latest version is FREE and EASY for current FlowJo v10 customers with a FlowJo Portal License. 

Simply download the latest version of FlowJo v10 below and sign in with your FlowJo Portal ID. 


Download previous versions of FlowJo™ Software here. [add url]

Click here to learn more about running FlowJo on different operating systems. [add url https://docs.flowjo.com/flowjo/getting-acquainted/version-compatibility/]

Dongle Questions?

If you would like more information about your dongle, please call 1-541-201-0022 or email license support with the dongle serial ID of your USB dongle. Instructions for locating your dongle serial ID can be found here under Locating your Dongle ID.


Important notes about dongle upgrades

  • We do not offer discounts on upgrades
  • If you are unsure of which version your dongle allows access to, call 1-541-201-0022 or email flowjooffice@bd.com for license support.
  • If your dongle is in good working condition, we can upgrade it electronically. We also offer physical upgrade and replacement which includes a shipping fee.
  • If you wish to turn in your dongle towards FlowJo Portal credit, we will use the original purchase date of your dongle.
  • Upgrades do not qualify for dongle trade-in credit towards a FlowJo Portal license.
  • Visit our FAQ page [add link]