Below is a selection of our full list of new features and fixes in 10.0.7. For an exhaustive list, please see this page.
Fixed:
10.0.7r2:
- Compensation is now “remembered” in templates
- Compensation population size restriction has been reduced to a warning if under 5%.
- Manual gating will override Compensation Matrix calculation and allow matrix to calculate if it exists
- Cytek data transforms fixed
- Miltenyi fcs 3.1 concatenate export files issue resolved.
- 10.0.6 workspace opened in 10.0.7 could drop parameters bug resolved
- Cosmetic fixed including dialogs and tip text rollovers have been improved.
- Several broken links to docs were fixed.
- Several minor stability improvements
- Improved functionality of Linux Version
10.0.7:
- License dialogs have been simplified.
- Data Scaling:
- Compensation matrix reading and data scaling for Miltenyi MACSQuantify-exported MQD and FCS3.1 have received numerous improvements. There is now a custom transformation available for Miltenyi MQD data, available from the T-Button, though note that no configuration is possible with this transform
- Data scaling for multiple Sony data types is improved including the EC800 and data from the SP6800 Spectral Analyzer
- Scaling improvements for Accuri FSC and SSC
- Arcsinh transformation for improved CyTOF data display
- IsFloC Custom transformation
- Boolean Gates:
- Gate creation using any boolean gate selection
- Combination gates
- Statistics after setting a NOT gate at the grandchild level
- Compensation Matrix Editor:
- Improved feedback for edits to matrices and gates, as well as tooltips added to compensation windows.
- Overlay uncompensated with preview
- Visualization of positive gate overlays
- Compensation with MoFlow and Cytek data files
- Reset parameter
- Derived Parameters and their transforms, and saving derived parameters.
- Export:
- Save as Excel
- Group export of sample data
- Concatenation of Beckman Coulter files
- Copying histograms to Excel
- File Formats: Opening FlowJo 9.7.1 .jo files
- Graph Window:
- Deleting gates with child gate open
- Opening compensation group-gated samples
- Curly quad resizing visualization
- Gate annotation on right edges
- Rectangle gate increase/decrease size using +/- buttons
- Control gate application
- Transposing of axes with spider, curly, and standard quadrant types
- Display of time parameter
- Layout Editor:
- Multiple improvements to grids
- Changing graph type and axes selections to multiple graphs
- Ancestry and Backgating selection updating and labeling
- Resolution of web pages batch outputs
- Outliers, image saving in PDF outputs
- Many fixes to saving and print dialogs
- Improvements to Layout Overlays
- Preferences:
- Compensation Cytometry, and Plate preferences/
- Workspace sort order preference
- Printing:
- Scaling tables to one page
- Header/Footer issues
- Avoiding page breaks
- Table Editor:
- Batching to printer
- Multiple improvements to batching to figure and to the Layout Editor
- Undo:
- Changing graph type
- Many fixes to LE graphic manipulation
- Moving quad gates
- Workspace:
- Dialog/message improvements across the program.
- Improved feedback and recommendations for memory management.
New Features:
- Platforms:
- A new Cell Cycle platform for univariate, bivariate, and multivariate analyses.
- Plate Editor : An entirely new platform for annotating, grouping, and organizing plate-based flow cytometry data including new tools for:
- Script Editor: A new platform which provides low level access to the core tools of the FlowJo engine.
- Compatibility: FlowJo is now available for LINUX.
- Gating:
- Workspace:
- Added ability for keywords to move to the top of the list once used
- Clicking on a compensation node opens compensation editor
- Changed name of “Instrumentation” band to “Cytometry” (workspace tab)
- Layout Editor :
- New sidebar for quickly editing the properties of a selected chart or object
- New Visualizations for Plate-based data including whole-plate heatmapping and Chernoff faces
- Table Editor:
- Batched report menu re-design as a well as a “Hide/Show Summary Stats” menu item
- Export:
- Parameter annotation added to CSV Export
- New feature to select to export compensated parameters into new FCS files
- Preferences:
- Printing and ruler preferences for the Layout Editor update live
- Added Preferences for default histogram style
- “Don’t show again” preferences for dialog windows
- Plate preferences for setting preferred visualizations and frequently-used keywords.
- Improved Experiment Annotation tool for MIFlowCyt annotation of experiments, included in the new Experiment band.
- Icon improvement in the workspace .
- Transforms are now saved with templates which preserves gating geometry and allows for the automated application of both transforms and gates.
Known issues:
- Multidimensional Cell Cycle Modeling is under construction and will be released soon in a beta version.
- 3D Viewer, Kinetics, and Population comparison are still under construction.
- Boolean combination gates containing NOT with AND or OR gates. Identical gates are not clone gates.
- Compensation with BioRad S3 samples.
- No progress indication when opening ACS files.
- PDF exporting when legend runs over a page.
- Opening 9.7.1 jo files: Bisector gate is converted to two range gates, and quadrant gates are converted to 4 rectangle gates.
- Layout Editor:
- FlowJo will run out of memory when batching NxN many times
- batched tables to the Layout Editor do not update
- We do not support Java 7. Windows versions include Java 6, and Java 6 can be installed with OSx along with Java 7 so FlowJo can use Java 6.
- Undo and Redo may have unexpected results. We hope this is better in a future release. If you have specific cases where undo or redo is failing, please let us know.
- Workspace:
- Insufficient progress when opening large WSP files…
- User not provided feedback that workspace is still calculating on long calculations
Tags: FlowJo