The following papers are great to learn more about compensation:
- Spectral Compensation for Flow Cytometry: Visualization Artifacts, Limitations, and Caveat. Mario Roederer, Cytometry 45:194 –205 (2001)
- A practical approach to multicolor flow cytometry for immunophenotyping. Baumgarth, Roederer. Journal of Immunological Methods 243 (2000) 77–97
- Seventeen-colour flow cytometry: unravelling the immune system. Perfetto, Chattopadhyay and Roederer. Nature Review, Immunology Vol. 4 Aug 2004 p. 649
- Nine Color Eleven Parameter Immunophenotyping Using Three Laser Flow Cytometry. Bigos, Baumgarth, Jager, Herman, Nozaki, Stovel, Parks, Herzenberg. Cytometry 36:36–45 (1999)
- New approaches to fluorescence compensation and visualization of FACS data. Tung JW, Parks DR, Morre WA, Herzenberg LA and Herzenberg LA. Clinical Immunology. 110:273-283 (2004)
The following links can provide additional information:
http://www.drmr.com/compensation/
http://flowcyt.salk.edu/howto/compensation/compensation-howto.html
http://lists.purdue.edu/pipermail/cytometry/2005-June/028944.html
Tags: FlowJo